Preliminary quantitative reverse-transcription (qRT)-PCR analysis ofc-myc transgene expression in the multiple organs was performed in RNA utilizing the TaqMan protocol (Invitrogen, Carlsbad, CA) customized for the ARR2PBKCRc-MycbGHpA transgene
Preliminary quantitative reverse-transcription (qRT)-PCR analysis ofc-myc transgene expression in the multiple organs was performed in RNA utilizing the TaqMan protocol (Invitrogen, Carlsbad, CA) customized for the ARR2PBKCRc-MycbGHpA transgene. We also used paraffin-embedded parts of several prostates extracted from Hi-Myc transgenic mice developed originally by Ellwood-Yen and coworkers (20) for Cav-1 immunostaining. == Immunohistochemical analyses of…