Cells were then stained with annexin V, 7-AAD, anti-CD20, anti-CD21, and G6 mAbs and were analyzed by circulation cytometry
Cells were then stained with annexin V, 7-AAD, anti-CD20, anti-CD21, and G6 mAbs and were analyzed by circulation cytometry. chronic antigenic activation of a limited pool of preexisting autoreactive B cells. We have proposed that persistently high levels of HCV-containing immune complexes stimulate the proliferation of RF-bearing B cells,6 but the exact antigen(s) and stimulatory…