The amplimers 5-GTTTTGTAAAGGGAGCCGAC-3 and 5-CCTGACTACTGAGCCTGGTTTCTC-3 were used to detect the allele (224 bp) and the allele (264 bp). 1figure product 4. This file contains raw source data used to make the graphs offered in Physique 1figure product 4.DOI: http://dx.doi.org/10.7554/eLife.18414.012 elife-18414-fig1-figsupp4-data1.xlsx (50K) DOI:?10.7554/eLife.18414.012 Figure 1figure product 5source data 1: Source data for Figure 1figure product 5. This file contains raw source data used to make the graphs offered in Physique 1figure product 5.DOI: http://dx.doi.org/10.7554/eLife.18414.014 elife-18414-fig1-figsupp5-data1.xlsx (45K) DOI:?10.7554/eLife.18414.014 Figure 1figure product 6source data JSH 23 1: Source data for Figure 1figure product 6. This file contains raw source data used to make the graphs offered in Physique 1figure product 6.DOI: http://dx.doi.org/10.7554/eLife.18414.016 elife-18414-fig1-figsupp6-data1.xlsx (54K) DOI:?10.7554/eLife.18414.016 Figure 1figure product 7source data 1: Source data for Figure 1figure product 7. This file contains raw source data used to make the graphs offered in Physique 1figure product 7.DOI: http://dx.doi.org/10.7554/eLife.18414.018 elife-18414-fig1-figsupp7-data1.xlsx (60K) DOI:?10.7554/eLife.18414.018 Determine 1figure product 8source data 1: Source data for Determine 1figure product 8. This file contains raw source data used to make the graphs offered in Physique 1figure product 8.DOI: http://dx.doi.org/10.7554/eLife.18414.020 elife-18414-fig1-figsupp8-data1.xlsx (261K) DOI:?10.7554/eLife.18414.020 Determine 2source data 1: Source data for Determine 2. This file JSH 23 contains raw source data used to make the graphs offered in Physique 2.DOI: http://dx.doi.org/10.7554/eLife.18414.022 elife-18414-fig2-data1.xlsx (44K) DOI:?10.7554/eLife.18414.022 Physique 3source data 1: Source data for Physique 3. This file contains raw source data used to make the graphs offered in Physique 3.DOI: http://dx.doi.org/10.7554/eLife.18414.024 JSH 23 elife-18414-fig3-data1.xlsx (39K) DOI:?10.7554/eLife.18414.024 Determine 4source data 1: Source data for Determine 4. This file contains raw source data used to make the graphs offered in Physique 4.DOI: http://dx.doi.org/10.7554/eLife.18414.026 elife-18414-fig4-data1.xlsx (39K) DOI:?10.7554/eLife.18414.026 Determine 5source data 1: Source data for Determine 5. This file contains raw source data used to make the graphs offered in Physique 5.DOI: http://dx.doi.org/10.7554/eLife.18414.028 elife-18414-fig5-data1.xlsx (32K) DOI:?10.7554/eLife.18414.028 Determine 6source data 1: Source data for Determine 6. This file contains raw source data used to make the graphs offered in Physique 6.DOI: http://dx.doi.org/10.7554/eLife.18414.031 elife-18414-fig6-data1.xlsx (57K) DOI:?10.7554/eLife.18414.031 Physique 6figure product 2source data 1: Source data for Physique 6figure product 2. This file contains raw source data used to make the graphs offered in Physique 6figure product 2.DOI: http://dx.doi.org/10.7554/eLife.18414.034 elife-18414-fig6-figsupp2-data1.xlsx (56K) DOI:?10.7554/eLife.18414.034 Physique 6figure product JSH 23 3source data 1: Source data for Physique 6figure product 3. This file contains raw source data used to make the graphs offered in Physique 6figure product 3.DOI: http://dx.doi.org/10.7554/eLife.18414.036 elife-18414-fig6-figsupp3-data1.xlsx (50K) DOI:?10.7554/eLife.18414.036 Determine 7source data 1: Source data for Determine 7. This file contains raw source data used to make the graphs offered in Physique 7figure product 1.DOI: http://dx.doi.org/10.7554/eLife.18414.038 elife-18414-fig7-data1.xlsx JSH 23 (50K) DOI:?10.7554/eLife.18414.038 Figure 7figure product 1source data 1: Source data for Figure 7figure product 1. This file contains raw source data used to make the graphs offered in Physique 7figure product 1.DOI: http://dx.doi.org/10.7554/eLife.18414.040 elife-18414-fig7-figsupp1-data1.xlsx (482K) DOI:?10.7554/eLife.18414.040 Abstract Arterial occlusive diseases are major causes of morbidity and mortality. Blood circulation to the affected tissue must be restored quickly if viability and function are to be preserved. We statement that disruption of the mixed-lineage protein kinase (MLK) – cJun NH2-terminal kinase (JNK) signaling pathway in endothelial cells causes severe blockade of blood flow and failure to recover in the murine femoral artery ligation model of hindlimb ischemia. We show that this MLK-JNK pathway is required for the formation of native collateral arteries that can restore circulation following arterial occlusion. Disruption of the MLK-JNK pathway causes decreased Dll4/Notch signaling, excessive sprouting angiogenesis, and defects in developmental vascular morphogenesis. Our analysis demonstrates that this MLK-JNK signaling pathway is Rabbit Polyclonal to SFRS5 usually a key regulatory mechanism that protects against ischemia in arterial occlusive disease. DOI: http://dx.doi.org/10.7554/eLife.18414.001 (EWT and.