of the positive was easily differentiated from negative serum with all three exposure times, variations in background reactivity with negative serum was minimal
of the positive was easily differentiated from negative serum with all three exposure times, variations in background reactivity with negative serum was minimal. Table 1 ELISA with recombinant nucleocapsid protein immobilized to wells in either bicarbonate or PBS buffer Conjugate dilutionBufferSera GrayArkConnMassNegBlank 500Bicarb1.361.121.480.600.1820.09PBS1.010.651.160.290.170.111000Bicarb1.231.041.150.410.100.06PBS0.800.500.650.210.120.052000Bicarb0.940.630.920.270.0730.05PBS0.670.380.540.150.10.054000Bicarb0.630.450.730.180.0690.05PBS0.530.260.480.110.080.058000Bicarb0.530.230.440.110.0580.06PBS0.360.190.250.070.060.0516?000Bicarb0.280.090.210.080.0560.06PBS0.220.130.170.060.060.05 Open in a separate window 3.4. (Qiagen, Chatsworth, CA) as a fusion…