Next the membranes were washed many times with TBST, and the proteins were visualized by incubation with substrate solution (0

Next the membranes were washed many times with TBST, and the proteins were visualized by incubation with substrate solution (0.33?mg/ml of nitro blue tetrazolium, 0.17?mg/ml of 5-bromo-4-chloro-3-indolyl phosphate in 100?mM TrisCHCl, pH 9.5, 100?mM NaCl and 5?mM MgCl2), prepared according to Leary et al. cells against damages induced by lead. The obtained results revealed, that…