For vehicle-pulsed, ACTH-pulsed, or constant-ACTH-pulsed, concentrated vehicle or ACTH (1 M) was added to wells at time 0, 60, and 120 moments, media was removed by aspiration at time 10, 70, or 130 moments, and cells were washed with 1 PBS and incubated either in serum-free media alone (vehicle-pulsed or ACTH-pulsed conditions) or in serum-free media containing 10 nM ACTH (constant-ACTH-pulsed) for 50 moments

For vehicle-pulsed, ACTH-pulsed, or constant-ACTH-pulsed, concentrated vehicle or ACTH (1 M) was added to wells at time 0, 60, and 120 moments, media was removed by aspiration at time 10, 70, or 130 moments, and cells were washed with 1 PBS and incubated either in serum-free media alone (vehicle-pulsed or ACTH-pulsed conditions) or in serum-free…